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pSCH2012
pSCH2012
規(guī)格:
貨期:
編號:B162620
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pSCH2012
商品貨號 B162620
Designations pSCH2012
GenBank Number

M98270

Species Pseudomonas aeruginosa (Schroeter) Migula
Depositors KJ Shaw
Vector
Construct size (kb): 3.150000095367432
Insert
DNA: cDNA
Insert lengths(kb): 0.2039999961853027
Gene product: aminoglycoside-4'-adenyltransferase [ant(4')-IIa]
Insert Size (kb) 0.204
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): HindIII--3.0; PstI--3.0; HindIII/PstI--2.9, 0.2.
The insert contains the following restriction sites (approximate kb from the 5' end): ClaI--0.01; PvuII--0.18
A single gel purification of the PCR generated probe is necessary since flanking regions will co-amplify with the gene specific sequence. Failure to do so often results in high backgrounds and false positives with clinical E. coli strains.
A hybridization probe may be generated using the following vector specific PCR primers: modified T3 = 5'-CCCCTCACTAAAGGGAACAAAAGCTG-3' and modified T7 = 5'-CGCGTAATACGACTCACTATAGGGCGAA-3'.
References

Shaw KJ, et alThe application of molecular techniques for the study of aminoglycoside resistanceIn: Shaw KJ, et alMethods in molecular medicine: molecular approaches for the diagnosis and investigation of bacterial diseasesTotowa, NJHumana Presssubmitted, 1996

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周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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