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172-12A4
172-12A4
規(guī)格:
貨期:
編號:B163602
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 172-12A4
商品貨號 B163602
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with a synthetic peptide with the sequence LGSGAFGTIYKG(C) corresponding to residues 138-149 of the v-erb B oncogene.
The 170 kd EGF receptor is weakly detected in immunoblot screening of A431 cells.
In addition, a protein of approximately 100 kd is detected in extracts of human brain.
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a synthetic v-erb B oncogene peptide
Cellular Products
immunoglobulin; monoclonal antibody; against a synthetic v-erb B oncogene peptide
Comments
Animals were immunized with a synthetic peptide with the sequence LGSGAFGTIYKG(C) corresponding to residues 138-149 of the v-erb B oncogene.
Spleen cells were fused with Sp2/0 myeloma cells.
In ELISA tests using synthetic peptides representing erb B, fes/fps, fgr, fms, src/yes, mil/raf, abl, mos, CDC28 and the catalytic and regulatory subunit of cyclic AMP dependent protein kinase, the antibody recognizes only the erb B sequence.
The 170 kd EGF receptor is weakly detected in immunoblot screening of A431 cells. In addition, a protein of approximately 100 kd is detected in extracts of human brain.
The IgG1 and IgG2b reactivities of the antibody with the v-erb B peptide (138 to 149) was confirmed by ELISA. IgG1 was strongly positive while IgG2b was negative.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1 and IgG2b
Name of Depositor National Cancer Institute
Deposited As mouse (B cell); mouse (myeloma)
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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