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1A3.1
1A3.1
規(guī)格:
貨期:
編號:B163613
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 1A3.1
商品貨號 B163613
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with G. muris trophozoite surfaces.
By immunofluorescence the antibody shows smooth surface fluorescence, staining of the flagella and aggregation of the trophozoites.
Derivation
Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Giardia muris trophozoites
Cellular Products
immunoglobulin; monoclonal antibody; against Giardia muris trophozoites
Comments
Animals were immunized with G. muris trophozoites.
Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells.
The antibody reacts with G. muris trophozoite surfaces.
By immunofluorescence the antibody shows smooth surface fluorescence, staining of the flagella and aggregation of the trophozoites.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%; NCTC 109 medium, 10%; (Add 1 mM oxaloacetic acid, 0.45 mM sodium pyruvate, 4 mM L-glutamine, 0.1 mM hypoxanthine, 0.01 mM thymidine and 10 units/ml bovine insulin to this mixture); horse serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG3; kappa light chain
Name of Depositor MF Heyworth
Deposited As mouse (B cell); mouse (myeloma)
References

Heyworth MF, et al. Generation and characterization of monoclonal antibodies against Giardia muris trophozoites. Immunology 68: 341-345, 1989. PubMed: 2592009

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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