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36.5
36.5
規(guī)格:
貨期:
編號(hào):B163717
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 36.5
商品貨號(hào) B163717
Organism Mus musculus, mouse
Tissue embryo
Cell Type pluripotent embryonic stem cell
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age embryo, blastocyst
Strain 129/Sv+c/+p
Applications
This line was derived by inserting a construct containing a disrupted murine Lyt-2 (CD8) gene.
After selection for neomycin resistance and presence of Lyt-2 sequences upstream of the insertion, the 36.5 cell line was established.
This line has been used to produce mice deficient in expression of CD8.
The cells can be maintained in the undifferentiated state by frequent subculture on feeder layers of irradiated (12000 rads) or mitomycin C treated (0.01 mg/ml for 90 minutes) primary mouse embryonic fibroblasts or STO cells.
Storage Conditions liquid nitrogen vapor phase
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
This line was derived by inserting a construct containing a disrupted murine Lyt-2 (CD8) gene.
Comments

This line was derived by inserting a construct containing a disrupted murine Lyt-2 (CD8) gene. 

The Lyt-2 genes was disrupted by insertion of a plasmid containing a neomycin resistance gene into the first exon of the Lyt-2 gene. 

After selection for neomycin resistance and presence of Lyt-2 sequences upstream of the insertion, the 36.5 cell line was established. 

This line has been used to produce mice deficient in expression of CD8.

The cells can be maintained in the undifferentiated state by frequent subculture on feeder layers of mitomycin C treated STO cells (MITC-STO ATCC 56-X.2) (see ATCC 56-X.2, MITC-STO cells).

Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 mM 2-mercaptoethanol, 85%; fetal bovine serum, 15%
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. The line should be grown on feeder layers of irradiated or mitomycin C treated (0.01 mg/mLfor 90 minutes) primary mouse embryonic fibroblasts or STO cells (see ATCC CRL-1503 or ATCC 56-X, irradiated STO cells).
  1. Remove and discard culture medium.
  2. Rinse the cell layer with 0.25% (w/v) trypsin-0.03% (w/v) EDTA solution. Remove the trypsin and incubate at 37°C until cells detach (approximately 10 minutes).
  3. Add complete growth medium and aspirate cells by gently pipetting.
  4. Add appropriate aliquots of the cell suspension to new culture vessels with fresh feeder layer cultures.
  5. Incubate cultures at 37°C.

Subcultivation Ratio: 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 days

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Name of Depositor Ontario Cancer Institute
Deposited As Mus musculus
U.S. Patent Number
References

Mak TW. Mutant mouse lacking CD8 surface marker. US Patent 5,530,178 dated Jun 25 1996

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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