久久久久国产成人精品亚洲午夜,成人免费毛片一区二区三区,国产成人久久精品激情91,国产成人无码精品久久久免费,精品欧美国产一区二区三区不卡,国产成人一区二区三区影院,国产精品久久久久久,久久久WWW成人免费精品,欧美91精品久久久久网免费

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > 3C9-D11-H11
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
3C9-D11-H11
3C9-D11-H11
規(guī)格:
貨期:
編號:B163734
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 3C9-D11-H11
商品貨號 B163734
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Derivation
Mice were immunized with purified PPV.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against porcine parvovirus (PPV)
Cellular Products
immunoglobulin; monoclonal antibody; against porcine parvovirus (PPV)
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Mice were immunized with purified PPV.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody has both virus neutralization and hemagglutinating activities.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; bovine calf serum, 2.5%
Subculturing

Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL.

Medium Renewal: Two to three times weekly
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG1
Name of Depositor RA Van Deusen
Deposited As mouse (B cell); mouse (myeloma)
References

8th Proc. Intl. Pig. Vet. Soc. Gent, Belgium: 1984.

Katz JB, van Deusen RA. Radioimmunoassay of adjuvant-associated porcine parvovirus using a monoclonal antibody in a nitrocellulose membrane system. J. Virol. Methods 12: 193-198, 1985. PubMed: 3009510

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
淮安市| 深圳市| 湘阴县| 府谷县| 普陀区| 车险| 涟水县| 丹阳市| 宝清县| 儋州市| 青州市| 武冈市| 石屏县| 湄潭县| 灵台县| 拉孜县| 永靖县| 太康县| 昔阳县| 荃湾区| 双峰县| 陵川县| 朝阳区| 新巴尔虎左旗| 鞍山市| 安义县| 呼玛县| 台南市| 尉犁县| 枝江市| 类乌齐县| 孝感市| 孟津县| 卓尼县| 泗水县| 衡阳市| 东乌| 梅河口市| 安仁县| 舞钢市| 宁夏|