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AF3-12.1.3
AF3-12.1.3
規(guī)格:
貨期:
編號:B163915
品牌:Mingzhoubio

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產品名稱 AF3-12.1.3
商品貨號 B163915
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against H-2 K k
Cellular Products
immunoglobulin; monoclonal antibody; against H-2 K k
Comments
Animals were immunized with spleen cells from A/J mice.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody does not react with the b, d, f, j, p, q, r, s, u or v H-2 haplotypes.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor AM Stall
Deposited As mouse (B cell); mouse (myeloma)
References

Loken MR, Stall AM. Flow cytometry as an analytical and preparative tool in immunology. J. Immunol. Methods 50: R85-R112, 1982. PubMed: 7047643

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
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