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Ig(1a)8.3 (formerly 20-8.3)
Ig(1a)8.3 (formerly 20-8.3)
規(guī)格:
貨期:
編號(hào):B164831
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) Ig(1a)8.3 (formerly 20-8.3)
商品貨號(hào) B164831
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with the Fc fragment, not the Fab fragment of mouse IgG2a, at a determinant probably located within the CH2 region.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the Ig-1a allotype on mouse IgG2a
Cellular Products
immunoglobulin; monoclonal antibody; against the Ig-1a allotype on mouse IgG2a
Comments
Animals were immunized with antigen - antibody complexes (BALB/c anti Bordetella pertussis with B pertussis).
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with the Fc fragment, not the Fab fragment of mouse IgG2a, at a determinant probably located within the CH2 region.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 20%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere ATCC tested fetal bovine serum is available as ATCC Catalog No. 30-2020.
  • Subculturing
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
    Isotype IgG2a
    Name of Depositor LA Herzenberg
    Deposited As mouse (B cell); mouse (myeloma)
    References

    Oi VT, Herzenberg LA. Localization of murine Ig-1b and Ig-1a (IgG2a) allotypic determinants detected with monoclonal antibodies. Mol. Immunol. 16: 1005-1017, 1979. PubMed: 94425

    Oi VT, et al. Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens. Curr. Top. Microbiol. Immunol. 81: 115-129, 1978. PubMed: 567555

    梅經(jīng)理 17280875617 1438578920
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    李經(jīng)理 13626845108 972239479
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