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LB 27.4 (LB 15.13)
LB 27.4 (LB 15.13)
規(guī)格:
貨期:
編號:B164958
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 LB 27.4 (LB 15.13)
商品貨號 B164958
Organism Mus musculus (B cell); Mus musculus (lymphoma), mouse (B cell); mouse (lymphoma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Lymphoma
Applications
The line was produced by fusing A20.2J lymphoma cells (I-Ad, I-Ed) with T cell depleted spleen cells from C57BL/10 mice (I-Ab, I-Eb).
Tested and found negative for ectromelia virus (mousepox).
Derivation
The line was produced by fusing A20.2J lymphoma cells (I-Ad, I-Ed) with T cell depleted spleen cells from C57BL/10 mice (I-Ab, I-Eb).
Comments
The LB 27.4 bears surface I-Ad,b/d,b and I-Ed,d/b molecules and can present antigen to appropriate I region restricted T cell hybridomas.
The line was produced by fusing A20.2J lymphoma cells (I-Ad, I-Ed) with T cell depleted spleen cells from C57BL/10 mice (I-Ab, I-Eb).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere
  • 0.05 mM 2-Mercaptoethanol

Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor P Marrack, J Kappler
Deposited As mouse (B cell); mouse (lymphoma)
References

Kappler J, et al. Antigen presentation by Ia+ B cell hybridomas to H-2 restricted T cell hybridomas. Proc. Natl. Acad. Sci. USA 79: 3604-3607, 1982. PubMed: 6980413

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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