| 產(chǎn)品名稱(chēng) |
Mab 2B5 |
| 商品貨號(hào) |
B165067 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Strain: BALB/c (B cell); BALB/c (myeloma) |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Animals were immunized with purified human lipopolysaccharide (LPS) binding protein (LBP) Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human lipopolysaccharide (LPS) binding protein (LBP) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human lipopolysaccharide (LPS) binding protein (LBP) |
| Comments |
Animals were immunized with purified human lipopolysaccharide (LPS) binding protein (LBP) The monoclonal antibody Mab 2B5 (ATCC HB-11491) recognizes overlapping epitopes on LPB whereas the monoclonal antibody Mab 1E8 (ATCC HB-11490) recognizes unique LBP epitopes. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG1; kappa light chain |
| Name of Depositor |
Scripps Research Inst. |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Kirkland T, et al. Monoclonal antibodies immunoreactive with lipopolysaccharide binding protein (LBP) and methods of their use. US Patent 6,232,080 dated May 15 2001
|
