久久久久国产成人精品亚洲午夜,成人免费毛片一区二区三区,国产成人久久精品激情91,国产成人无码精品久久久免费,精品欧美国产一区二区三区不卡,国产成人一区二区三区影院,国产精品久久久久久,久久久WWW成人免费精品,欧美91精品久久久久网免费

熱門(mén)搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > Mo [Mo T]
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
Mo [Mo T]
Mo [Mo T]
規(guī)格:
貨期:
編號(hào):B165181
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Mo [Mo T]
商品貨號(hào) B165181
Organism Homo sapiens, human
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2  [Cells contain Retrovirus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease hairy cell leukemia
Gender male
Storage Conditions liquid nitrogen vapor phase
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Clinical Data male
Genes Expressed
granulocyte macrophage colony stimulating factor (GM-CSF, GM CSF), also known as neutrophil migration inhibitory factor (NIF-T); interferon
Cellular Products
granulocyte macrophage colony stimulating factor (GM-CSF, GM CSF), also known as neutrophil migration inhibitory factor (NIF-T); interferon
Comments

A plasmid containing the DNA sequence coding from Mo that codes for CSF is available as ATCC 39754.

Mo cells contain a replication competent genome of Human T Cell Leukemia Virus II (HTLV-II) and two defective HTLV-II genomes.

For long term culture, the cells can be grown in medium with 2% fetal bovine serum.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
The cells are subcultured every 7 days by centrifugation and division of the cell pellet 1:4 (1:6 maximum). Do not dilute below 2 X 105 viable cells/ml or allow to exceed 2 X 106 cells/ml. Culture at 5% CO2.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6
Medium Renewal: Add medium every 2 to 3 days depending on cell density

Note:The donor suggests using Falcon flasks for optimum growth.

Cryopreservation
Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: 8 to 9% CO2 in air
STR Profile
Amelogenin: X,Y
CSF1PO: 10
D13S317: 9,13
D16S539: 11
D5S818: 11,12
D7S820: 9,12
THO1: 9.3
TPOX: 8,11
vWA: 17,18
Name of Depositor Genetics Institute, Inc., Regents of the University of California
U.S. Patent Number
References

Golde DW, Quan SG. Unique T-lymphocyte line and products derived therefrom. US Patent 4,438,032 dated Mar 20 1984

Clark SC, et al. Method for identification and isolation of DNA encoding a desired protein. US Patent 4,675,285 dated Jun 23 1987

Saxon A, et al. Immunologic characterization of hairy cell leukemias in continuous culture. J. Immunol. 120: 777-782, 1978. PubMed: 344797

Gasson JC, et al. Purified human granulocyte-macrophage colony-stimulating factor: direct action on neutrophils. Science 226: 1339-1342, 1984. PubMed: 6390681

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
普洱| 兴安县| 环江| 洪江市| 清水河县| 尉犁县| 察隅县| 梁河县| 大田县| 喀喇沁旗| 太原市| 台北市| 如东县| 梧州市| 南丹县| 平遥县| 南汇区| 喜德县| 长阳| 巫山县| 林甸县| 军事| 阜阳市| 奇台县| 吉安县| 七台河市| 资兴市| 扎鲁特旗| 乌拉特后旗| 宜城市| 西安市| 七台河市| 宜黄县| 棋牌| 麻栗坡县| 留坝县| 武乡县| 司法| 冷水江市| 皋兰县| 光泽县|