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These cells are grown in Dulbecco?s Modified Eagle?s Medium/Ham?s Nutrient Mixture F-12, 1:1 with 2.5 mM L-glutamine, 1.2 g/L sodium bicarbonate, 15mM HEPES and 0.5mM sodium pyruvate.

Supplemented with:

• 10% fetal bovine serum
• 10 µg/ml insulin
• 5.5 µg/ml transferrin
• 5 ng/ml selenium
• 40 ng/ml dexamethasone

This medium is formulated for use with a 5% CO₂ in air atmosphere.

ATCC modified and tested medium formulation (without the additional supplements or serum described above) is available as ATCC 30-2006. 

ATCC tested fetal bovine serum is available as ATCC 30-2020 (500ml) and ATCC 30-2021 (100ml).