| 產(chǎn)品名稱 |
Rat2 |
| 商品貨號(hào) |
B165577 |
| Organism |
Rattus norvegicus, rat |
| Cell Type |
fibroblast |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
normal |
| Age |
embryo; fetus |
| Strain |
Fischer |
| Derivation |
This line was derived from the a 5-bromo-2'-deoxyuridine resistant strain of the Fischer rat fibroblast 3T3 like cell line, Rat1 (developed by W.C. Topp). |
| Comments |
Rat2 lacks detectable nuclear thymidine kinase, is highly transfectable by exogenous DNA and is phenotypically normal. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Twice per week |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C |
| Name of Depositor |
B Ahrens |
| Deposited As |
Rattus sp. |
| References |
Topp WC. Normal rat cell lines deficient in nuclear thymidine kinase. Virology 113: 408-411, 1981. PubMed: 7269249
Peng M, et al. Partial inhibition of Na+/K+-ATPase by ouabain induces the Ca2+-dependent expressions of early-response genes in cardiac myocytes. J. Biol. Chem. 271: 10372-10378, 1996. PubMed: 8626609
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