It is important to check whether the strain is [rho+] or [rho-] prior to use for complementation analyses.
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Type Strain
no
Genotype
MATa pet1 arg4 thr1 trp5-48 his5-2 lys1 ade2-1 gal 2
RefSherman F, Slonimski PP. Respiration-deficient mutants of yeast. II. Biochemistry. Biochim. Biophys. Acta 90: 1-15, 1964. , (Beck J, et al. Autonomy and biogenesis of mitochondria and chloroplasts. : North Holland/American Elsevier; 1971.), (galactose-sensitive due to pet gene)
Preceptrol®
no
Mating Type
a
Ploidy
haploid
Comments
Respiration: uncharacterized nuclear petite genes (pet) (CE1)
Genetic petites become [rho-] at a high frequency. It is important to check whether the strain is [rho+] or [rho-] prior to use for complementation analyses.
Medium
ATCC® Medium 1069: YPAD medium
Growth Conditions
Temperature: 25.0°C
Atmosphere: Typical aerobic
Name of Depositor
YGSC
Special Collection
Yeast Genetic Stock Center
Chain of Custody
ATCC <<--YGSC<<--S. Fogel
References
Sherman F, Slonimski PP. Respiration-deficient mutants of yeast. II. Biochemistry. Biochim. Biophys. Acta 90: 1-15, 1964.
Beck J, et al. Autonomy and biogenesis of mitochondria and chloroplasts. : North Holland/American Elsevier; 1971.
galactose-sensitive due to pet gene
Sherman F, Slonimski PP. Respiration-deficient mutants of yeast. II. Biochemistry. Biochim. Biophys. Acta 90: 1-15, 1964.
Beck J, et al. Autonomy and biogenesis of mitochondria and chloroplasts. : North Holland/American Elsevier; 1971.