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lambdaA6 [pcD-GALT]
lambdaA6 [pcD-GALT]
規(guī)格:
貨期:
編號:B244283
品牌:Mingzhoubio

標準菌株
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DNA
RNA

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凍干粉
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甘油
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產品名稱 lambdaA6 [pcD-GALT]
商品貨號 B244283
Designations lambdaA6 [pcD-GALT]
GenBank Number

X07208

Species Homo sapiens, human
Vector
Construct size (kb): 45.5
Insert
DNA: genomic
Insert lengths(kb): 16.0
Tissue: AT5B1 line (T-cell chronic lymphocytic leukemia)
Gene product: T cell receptor, gamma, variable region V9 [TCRGC1]
Alleles: A2, A1
Insert Size (kb) 16.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): SacI--20.0, 6.3, 0.9; SmaI--19.0, 9.2, 6.0, 5.2, 4.6; XbaI--20.0, 12.0, 9.1, 3.2; XhoI--20.0, 16.0, 9.1; KpnI--17.0, 15.0, 7.2, 4.0, 1.5.
The insert contains the following restriction sites (approximate kb from the 5' end): SacI--6.3, 15.3, 16 (polylinker site).
Contains the pseudogene VgammaA, a non-productive Vgamma9-Jgamma1 rearrangement, and exon 1 of TCRGC1. The 5' end begins about 8 kb upstream of Vgamma9 and extends about 7 kb beyond J1.
Additional GenBank/EMBL accession numbers: X08086, M12957.
References

LeFranc MP, et al. Diversity and rearrangement of the human T cell rearranging gamma genes: nine germ-line variable genes belonging to two subgroups. Cell 45: 237-246, 1986. PubMed: 2938743

Marie-Paule Lefranc, personal communication

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